Jai krishna Mahto

8 Questions 3 Answers 0 Followers

Questions related from Jai krishna Mahto

Restriction digestion product is smear. what is the cause behind it?

I digested bacterial plasmid with EcoRI and EcoRV. digested product was smear. But the plasmid has only two RE site.

01 December 2016 7,930 5 View

Whether pathogenic bacteria can be utilized for bioremediation purpose?

Klebsiella Pneumoniae, Pseudomonas aeroginosa isolated from environmental sample is showing very fast biphenyl degradation but these are pathogenic.

26 September 2016 5,685 7 View

Which medium to use for slow growing bacteria?

I wanted to isolate slow growing bacteria from environmental sample but LB is giving only few bacteria which has high doubling time.

26 September 2016 3,897 6 View

How to find degradation pathway from metabolites?

I got three peaks in the GC-MS data after running hexane extract of biphenyl degradation culture. first peak: 6H-[1,2,4]Triazolo[1,5-a]indole, 4a,5,7,8,8a,9-hexahydro-9-methylene, Phenol...

26 September 2016 7,799 1 View

Can we run hexane extracted sample in HPLC with acetonitrile as mobile phase?

I want to run HPLC for the hexane extracted sample. which mobile phase I should use?

09 September 2016 4,407 10 View

Why GC-MS data shows two peaks for same compound?

GC-MS data is given in the attachment. PFA

24 August 2015 1,733 17 View

What is the concentration of sodium pyrophosphate that should be used for cell dislodging from environmental soil sample?

Sample is from acid mine drainage.

17 June 2015 6,615 0 View

How can we perform anaerobic growth experiment when anaerobic work station is not working?

Reagents such as Resazurin, Cys-HCl are available with us.

02 June 2015 7,573 4 View