Advantages and disadvantages of gel elution and PCR clean up kits/Exo sap-IT Clean ups?
gel purification will always work in purifying away the pcr primers but is more time consuming and less efficient if you are running a large number of samples. Exo sap is excellent and quick but there is a group of primers that are resistant to exonuclease treatment so if one of your pcr primers is not degraded then you will be sequencing with 2 primers present so very occasionally sequencing can be poor
Dear Kurian,
You can refer to my PhD thesis for more information about PCR Clean up methods.
Good Luck
Nibas
gel purification will always work in purifying away the pcr primers but is more time consuming and less efficient if you are running a large number of samples. Exo sap is excellent and quick but there is a group of primers that are resistant to exonuclease treatment so if one of your pcr primers is not degraded then you will be sequencing with 2 primers present so very occasionally sequencing can be poor
I am agree with Paul ,Exo Sap is very good for few samples of sequences and I have got good sequences using Sanger .
- Agree with above answers about SAP EXO treatment
- In addition much cheaper than column/gel purification
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