How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How accurate is the shear modulus measured using PROCEQ DPC transducer of 40kHZ frequency on 100mm concrete cube specimen?

It is required to evaluate shear modulus of concrete cube specimen of 100mm size. Since the diameter of DPC transducer is around 84mm, I would like to know whether it can give reliable shear wave...

02 February 2021 1,052 3 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Do I need to transfect FuW-tetO-MCS and FUW-M2rtTA plasmids together in HEK293T or separately?

I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in...

12 January 2021 8,501 2 View

How to identify Peptide Concentration?

Any easy method to find custom peptide concentration? We have some synthesized peptides for our biotechnology and biochemistry based research, i am looking to find it's concentration. Anybody can...

04 January 2021 8,505 8 View

Best time-point to determine changes in mRNA via qPCR?

I am trying to determine what best time-point to harvest my cells, HMVEC, after transduction with long non-coding RNA to determine changes in mRNA levels, specifically VEGFR2/KDR. I am capable of...

19 December 2020 4,865 2 View

Is there any way to calibrate transducer without needle hydrophone?

Is there any way to calibrate transducer without needle hydrophone? Also any one know low cost hydrophone as precision acoustic is quite costly.

16 December 2020 1,070 2 View

How to convert mV output into MPa?

I am working on acoustic characterization of microbubbles using two transducers arranged at 90 degrees. During calibration, I am receiving response in dBm or mV. How I can know the PNP of my...

14 December 2020 1,814 5 View

Modeling and Simulation of Radial Piezoelectric Transducer?

I need some one working in comsol

10 December 2020 8,442 3 View

Transduction of mouse primary neurons with AAVs, protocol?

Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...

10 December 2020 7,900 2 View

Hi can anyone recommend a synovial cell line?

Hi, I am looking for recommendations for a synovial cell line that is transfectable and transducable. We are currently not interested in working with primary cells. Thank you in advance....

01 December 2020 1,894 3 View