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My recombinant protein can be expressed in E. coli but not in lactobacillus. Why?

I transformed a strain of lactobacillus using electroporation to introduce a constitutive expression vector (P-erm is the promoter) expressing a recombinant protein. The construct was first...

29 November 2023 4,541 3 View

Does addition of L-arginine into DMEM increases the production of nitric oxide by RAW264.7 cells after LPS treatment?

If yes, what is the ideal concentration of L-Arginine in DMEM?

04 October 2023 1,856 0 View

How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)?

How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)? I will need to determine whether the compound is paramagnetic of diamagnetic

03 August 2023 6,535 2 View

How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)?

How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)? Are there any good references? Thanks in advance.

03 August 2023 8,057 0 View

Anyone with experience analysing THP-1 derived dendritic cells using flow cytometry?

I encountered the problem of no signal detected from the flow analysis of my THP-1 derived dendritic cells (DCs). I have observed the morphology of my cells resembling that of DCs after...

05 June 2023 2,553 1 View

Is it feasible to perform RAD-seq with DNA isolation from mixed individual samples?

I would like to perform RAD-seq on one zooplankton with a body-size between 0.5-1cm. However, the DNA extracted from each individual by chelex can not work for library preparation. So I am...

02 June 2022 4,051 2 View

What is the mechanism for the formation of storms in Western Europe and Central China last summer?

Just in the past summer, in Germany and other western European countries, once in 400 years large storms hit the region and caused casualties and significant property loss. Similarly in the...

28 August 2021 6,820 0 View

How to deal with high lambda for Multi-trait GWAS analysis?

Hello everyone, I have conducted a multi-phenotype GWAS analysis. I have 5 phenotypes in total, the GWAS lambda ranged from 0.8 to 1.12, only two of them are acceptable (0.985, 1.030). Is there...

09 August 2021 6,306 0 View

What are the ways to seal my in-house prepared media glass bottles?

I prepare in-house media in Duran glass bottles, and am trying to find a way to seal them to prevent any tampering. I currently use shrink plastics post-sterilisation (either autoclaving or filter...

09 June 2021 8,105 1 View

Is it reasonable to use stress boundaries to simulate situations with tectonic stress in FLAC3D?

Dear all I want to build a small fault model, considering the tectonic stress, the horizontal stress is relatively high.Is this the case where you can only use the stress boundary.This is a fixed...

13 July 2020 557 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View