Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

How to calculate trapping parameter of the deconvolution peaks?

28 July 2020 2,413 2 View

Have any professors ever done research about the expression of fusion proteins in Drosophila S2 cells?

1. How to scale up the expression of fusion proteins? 2. How to improve the purity of fusion protein to 90%? I have tried affinity chromatography, but the purity by SDS-PAGE was not satisfied....

04 November 2019 8,479 6 View

How to calculate the active area of CNTs modified glassy carbon electrode?

"The active area of so porous electrode as the authors used could not be correctly evaluated using the Randles-SEVCIK formula. Just because the depletion layers of active places may overlap." How...

28 October 2019 1,782 3 View

Future of bioinformatic analysis in plant science ?

please share your idea regarding the future of bioinformatic analysis in plant science? recommend the preferred PC and MAC programs for the beginner ,and for the advanced users? as well as any...

24 October 2019 8,814 4 View

Bad performance of RQ-PCR standard curve constructed by plasmid

Hi guys! Recently I encountered a problem about the Albumin (ALB) standard curve which was used for normalizing my samples. The ALB standard curve was constructed by serial dilutions of plasmid...

25 July 2017 8,762 3 View

Recommendation for instrument and method to produce the macro-structure of weld joints?

Detailed information of the equipment (i.e. name, company, price, working mechanism) is highly appreciated.

08 September 2016 6,402 0 View

How can I get the free GIS data of China?

16 May 2015 3,254 2 View

How to prepare the solution for ternary CdSexTe(1-x) thin films??

Can anyone explain in brief how to prepare the solution for ternary CdSexTe(1-x) thin films e.g. CdSe(0.6)Te(0.4) thin film?

06 October 2014 4,718 2 View

Can anyone suggest a reference electrode for electrodeposition?

Can anyone suggest a reference electrode for electrodeposition in aqueous solution? Which one is better between a Saturated Calomel Electrode (SCE) and an Ag/AgCl reference electrode?What is the...

28 April 2014 1,889 21 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View