How to evaluate motivation in scholars, teachers and students who work from home during pandemic?

Dear all, What tool can be used to measure motivation in scholars, teachers and students who are in home isolation or work remotely? Any specific tool suitable for all people involved in Academia...

08 December 2020 7,968 10 View

How to replace the liquid blocker?

Dear colleagues, In my immunofluorescence stainigs I use liquid blocker pen, before solution with 0.3% Triton X-100. This helps to avoid dripping of solutions with primary antibodies and Triton...

30 September 2020 9,134 4 View

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06 July 2020 4,133 2 View

How long can one store primers?

Dear colleagues, one year ago we put our liquid primers in a high concentration into a -80 fridge. Can we still use them for the qPCR?

11 May 2020 4,521 16 View

What is an acceptable small sample size to carry out principal component analysis for a rating scale with 16 items???

Hi Can someone tell me what would be an acceptable sample size for principal component analysis for a rating scale with 16 items. Thanks!

30 March 2020 9,756 3 View

How to insert image in introductory text of survey in Opinio?

Hi I need some technical help. I am trying to insert an image in the introduction of a survey in Opinio. I click on 'insert image' button, then I am prompted to paste the url of the desired image,...

28 March 2020 1,543 3 View

How does the Excel work with GRG algorithm?

I'm struggling with my qualification work. Excel methods solve the math problem easy, but I don't catch the idea how he does it. I need to understand it to create the code or just to describe the...

12 March 2020 6,567 6 View

What to do if Fatal error: Atomtype LC3 not found and force field is fine?

When I was simulating my protein, "Fatal error: Atomtype LC3 not found" had occured. I checked my force field atom type and it contains LC3 with correct parameters. Before this error I used...

10 March 2020 2,189 2 View

Has anyone optimized ViromerRED in THP-1, H9, and MCF7 cell lines?

I am currently attempting to optimize ViromerRED transfection in THP-1, H9, and MCF7 cells. Our initial transfections with the first batch was promising, but the new viromer we received doesn't...

15 October 2019 5,053 4 View

Can anybody explain why does the line on gel disappear after 15 cycles of PCR?

We make test PCR to choose optimal quantity of cycles and amount of cDNA for amplification, and there are lines on 9 cycles and 12 cycles (same sample), but the line on 15 cycles disappear (also...

02 April 2019 3,158 7 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

What is the most suitable experimental design?

I am planning to do a study with the help of 45 farmers who are going to produce hybrid chilly seed for the first time, and I want to measure in what way semi-automated greenhouse will affect on...

14 January 2021 2,799 4 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

Greenhouse Simulation using COMSOL Multiphysics ?

Have anybody tried to simulate greenhouses using COMSOL Multiphysics? and is it doable?

26 December 2020 803 1 View

Isolation protocol for rice rhizosphere microbes?

I want to extract various microorganisms from the rice rhizosphere, particularly bacteria and fungi. Can anybody recommend an isolation protocol specific to the said purpose?

19 December 2020 3,541 3 View

How to find at which time point there is significance and between which groups in a repeated measures ANOVA?

I have conducted a repeated-measures ANOVA and a Tukey post hoc test. The data I have is for 3 seedling mutant lines, I am comparing their root growth over a timecourse of 19 hours. Within each...

13 December 2020 9,868 5 View

How could you explain that weather is a factor with such contribution in keeping the same level of GHG emissions?

" The IEA report looks at changes in greenhouse gas emissions between 2018 and 2019. It finds that energy-using activities increased in 2019, but the emissions did not increase because of a...

03 December 2020 7,073 3 View

Why do the allelopathic produce a positive effect?

Following an experiment where Zea mays and Cucurbita pepo seeds were planted individually and together, results showed a positive allelopathic effect on germination and seeling lenght as both...

03 December 2020 1,875 6 View

Does solar activity have an higher effect on climate change than CO2?

I started a research on analyzing some 23 indicators which are all somewhat related to influence climate change. The objective of my research is relating all indicators together by considering the...

30 October 2020 8,783 51 View

Are any Ph.D. programs or research labs working on Extraterrestrial habitat and greenhouse?

I am looking for opportunities to expand my research. Please let me know if you have any information. Just a note, I am available for collaboration on papers and projects.

19 October 2020 5,887 1 View