I am constructing bispecific antibody but having difficulty of finding the IgG Fc sequence for my construction. Where I could find it? Thx.
IMGT http://www.imgt.org
"IMGT®, the international ImMunoGeneTics information system® http://www.imgt.org, is the global reference in immunogenetics and immunoinformatics, created in 1989 by Marie-Paule Lefranc (Université de Montpellier and CNRS). IMGT® is a high-quality integrated knowledge resource specialized in the immunoglobulins (IG) or antibodies, T cell receptors (TR), major histocompatibility (MH) of human and other vertebrate species, and in the immunoglobulin superfamily (IgSF), MH superfamily (MhSF) and related proteins of the immune system (RPI) of vertebrates and invertebrates. IMGT® provides a common access to sequence, genome and structure Immunogenetics data, based on the concepts of IMGT-ONTOLOGY and on the IMGT Scientific chart rules. IMGT® works in close collaboration with EBI (Europe), DDBJ (Japan) and NCBI (USA). IMGT® consists of sequence databases, genome database, structure database, and monoclonal antibodies database, Web resources and interactive tools."
If you are using commercial antibodies it may be difficult to find the sequence. The companies don't like to let that info out. If you want to license the antibody then they may be more amenable.
Of course, for recombinant antibody constructs, you may want to start with the protein sequence and optimise the codon usage for the intended expression host rather than using a natural nucleotide sequence.
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