Where does transcription factor bind (Major groove or minor groove); please send the reference of the paper too.
Mostly TFs binds to major groove of double stranded structure, unless TFs is a TATA box binding protein, which binds in minor groove.
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
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