Where can we get the details about the types of experimental designs?

More Ihsan Ullah Khan Altaf's questions See All

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Are you looking for research collaboration ?

we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]

29 July 2024 6,626 0 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

15 July 2024 9,065 4 View

Can we calculate the CO2 adsorption capacity(mmol/g) of a solid adsorbent (MOF) using CO2_TPD analysis?

what is the relationship b/w TCD signal(a.u) and CO2 desorbed (mmol/g) ? can we plot CO2 desorbed(mmol/g) Vs Time(min) from TPD data?

15 July 2024 3,248 1 View

How to wash capsule filters (Membrane: polyethersulfone; pore size0.2u/0.45u) after filtration. can I wash by NaOH? if yes, what will be the molarity?

11 July 2024 8,969 1 View

Can any one send/provide me JCPDS card No. 53-0780 Please ?

Need JCPDS card No. 53-0780 if any one has access please . Thanks

26 June 2024 9,461 2 View

Is Anti-FLAG® M2 Magnetic Beads work for purification of 3x FLAG tagged protein ?

Can anyone share their experience of working with Anti-FLAG® M2 Magnetic Beads used for purification of 3x FLAG tagged protein.

25 May 2024 7,522 1 View

What impact will Ebrahim Raisi’s death have on Iran’s domestic and foreign policies? Are there any potential security implications following hs death?

Impact on Iran’s Policies: Domestic: Raisi’s death may lead to shifts in Iran’s political landscape. Foreign Policy: Immediate changes are unlikely, as the supreme leader holds final...

20 May 2024 3,143 3 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

What is best method for isolation bacteriophages, for Bacillus?

Recently, The isolation of bacteriophages from sewage water is so difficult, How to obtain phages plaques when i make isolation bacteriophage experiments for these bacterial types as bellow; 1-...

07 June 2024 9,473 1 View

How i can download sequences from MLST C. albicans database?

I need to download the sequence of seven housekeeping genes (i.e., AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b) of ST C. albicans to do phylogeny tree

18 October 2023 5,304 4 View

OneLambda Micro SSP HLA Class I DNA Typing Tray Worksheet interpreting?

Has anyone used the OneLambda Micro SSP Generic HLA Class I DNA Typing Tray (SSP1L) and can help me understand how I'm supposed to under the worksheet?

16 October 2023 3,096 0 View

How to generate clonal complex, do eburst analysis and phylogeny via Grape tree from E. Coli MLST sequence?

Hi please can anyone guide me on how to retrieve clonal complex (CC) from MLST sequence of seven house keeping genes of E. Coli, and also to do spartial distribution of the CC. Also, due to...

14 September 2023 5,256 1 View

Does anybody know how to do detect HLA A1?

Hi everyone, Im going to do HLA typing on the PBMCs to match our cancer cell lines which have been HLA typed. Most of them carry A02:01 and A01:01, some of them carry A29:02 and A24:02. I have...

16 July 2023 8,297 0 View

Could someone suggest me some alternative programs to START 2?

Sequence type analysis and recombinational tests (START2) I have information from MLST and I would like to analyze it

28 May 2023 9,550 0 View

Can anyone help me to understand MLST results?

I have MLST results in an excel sheet and want your help to understand how can I interpret MLST results. Here is a screenshot.

12 February 2023 1,964 2 View

I have some house keeping genes a part of these genes have been sequenced for MLST,how I can do gene assembly?

I need to know the name of a program to do genome assembly for each of these genes for each isolate

02 February 2023 663 2 View

Which website can download start2 software?

In the course of my research, I found that I needed to use the start2 (Sequence Type Analysis and Recombinational Tests Version 2) software. However, the original website...

30 October 2022 1,270 6 View

Juan Brines Solanes

You can try to get the book:

Experimental Designs, 2nd Edition

William G. Cochran, Gertrude M. Cox

ISBN: 978-0-471-54567-5. Wiley, 1992.

Muhammad Zahid Latif

Different books and lot of relevant material is available on line. Book of preventive medicine by K Park and Public Health by Maxy can be helpful