How i can download sequences from MLST C. albicans database?

More Wafa Rhimi's questions See All

Optimization of tetragonal structure ?

Hi all, What are the steps to optimizing a tetragonal structure using Wien2k code?

30 March 2024 9,205 0 View

Good greeting What are the steps to estimate the panel svar model in EViews 13?

good greeting What are the steps to estimate the panel svar model in EViews 13?

16 March 2024 9,251 1 View

Are increased LOD and LOQ values normal using LC-MS/MS?

Plant extracts were quantified utilizing LC-MS/MS in conjunction with SIM (selected ion monitoring). I anticipated obtaining extremely low values since the LOQ represents the lowest detectable...

29 January 2024 2,500 5 View

Dear All, kindly I want two protocols: 1. Polymorphonuclear leukocytes (PMNs or neutrophils) extraction from blood. 2. Degranulation of PMNs?

The objective: To compare the antimicrobial peptides compositions in neutrophils with stimulation and without stimulation.

19 September 2023 1,621 2 View

Hello, How to test the mechanism of action (biochemical point of view) of a anticancer-approved agent?

there are many agents approved to have anticancer agent using MTT or any other type of tests but yet the mechanism of how they induce anti proliferation or apoptotic activity is not reveled, So...

19 March 2023 2,169 3 View

How to improve the RNA concentration extracted from frozen human plasma samples ( not stored in trizol) (stored at -80)? Share your protocol if any?

I have extracted RNA from frozen human plasma samples by thermoscientific genejet RNA purification kit but of very poor quality. How to improve this extraction to run PCR for that. Attaching the...

23 January 2023 7,516 5 View

How to deploy the algorithm of psychoacoustic model MPEG 1?

I want to perform the psychoacoustic model of MPEG 1 to obtain the global masking threshold of a speech signal. Assuming that the spesch signal frame is of length N, i want to get the...

14 August 2022 7,565 0 View

Is there a way to calculate the equivalent dose for vitamin c in rats to be used for quails ?

We are aiming to use vit. c as antioxidant in quails. we are trying to set the effective dose for it. last papers used vit. c mixed with food for quails or vit. c for rats by gavage. How can we...

28 June 2022 9,107 0 View

Do you know any questionnaire that is valid, reliable and measures the level of physical activity for adolescents'?

Our team is looking for more questionnaires that we can use in our research. If you know any please let me know THANK YOU

15 February 2022 9,501 3 View

Do I have to do the whole process of data analysis after piloting interviews' questions?

Good morning. I wonder if you can help me answer this question. I'm doing a mixed method research. I'm required to pilot the interviews before conducting the actual ones. The question is: Do I...

18 December 2021 5,108 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?

27 July 2024 5,455 2 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Can biogeographical regions work as a character in a character matrix for a phylogenetic analysis?

Basically I'm helping with the matrix using a cosmolitan group, could one of the characters used for be biogeographical regions (Neartic, Neotropical, etc)? Most of the data in this matrix refers...

25 July 2024 9,753 2 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Younes Laidoudi

Dear Wafa,

You can download the sequences of MLST profiles from here: https://pubmlst.org/bigsdb?db=pubmlst_calbicans_seqdef&page=downloadAlleles

Wafa Rhimi

THANK YOU FRIEND

Harish Menghwar

Download it from here:

https://pubmlst.org/bigsdb?db=pubmlst_calbicans_seqdef

THANK YOU Dr Harish