Where from can I get cultures of R solani from different localities in India? I also need different rice pathogens like P. oryzae, H. oryzae, S oryzae, Sarocladium oryzae. Xanthomonas oryzae pv oryzae and other pathogens?
You can get some soil samples, and use baits like eucalyptus rods. This article (Link above) can be usefull for you.
http://www.scielo.br/scielo.php?pid=S0100-41582007000200004&script=sci_arttext
very easy to isolate from root rots of vegetables, sheath blights of rice etc.the fungus will not sporulate and will form sclerotia. Thick mucelium, with right angled branches. the culture is usually dull white to greyish and tend to turn brownish, remember sclerotia are not well defined round like others. They are crust like irregular shaped.
If you want cultures of R solani then you must contact Dr. CD Mayer, who is a faculty at Division of Plant Pathology, IARI New Delhi. He is working on this pathogen and has a collection of all over India. Contact him for cultures along with international VG groups. You may need them also in case you are planing to do compatibility groupings. As for other pathogens are considered. There are many scientists working on rice blast (Dr T R Sharma, Director NRCPB) and DRR Hyderabad. Take help from your Director (Dr. T Mahaptra). He is renowned scientist and can get cultures easily. Also look at ITCC, IARI and Chandigarh IMTech. They have collection. Also MAU.
Hopefully you will get these cultures
You can get the culture of Rhizoctonia solani (pathogenic on forest nurseries) from National Type Culture Collection (NTCC), Forest Pathology Division, Forest Research Institute, Dehradun.
Very good suggestion, typical culture of any fungi or bacteria can be collected officially from national culture bank, sometimes called herbarium. CMI was the world preservation center for fungal cultures while they were giving service to scientists working with systematic mycology or students required for identification of the isolated cultures. Noe many countries have their own culture bank. For isolation of rice pathogens you mentioned, you need special culture specific for each fungus. You can consult any plant pathology book.
Sir, you can get the pure culture of R. solani from the Microbial Type Culture Collection & Gene bank (MTCC), a unit of Institute of Microbial Technology (IMTECH), Chandigarh.
Manoj,
I am in regular touch with MTCC even I have deposited numbers of cultures there but R. solani from rice is not available there.
Division of Plant pathology has ITCC (Indian Type Culture Collections) and they maintain them. If you need you can contact the Mycologists Dr.Deeba Kamil, Dr.T.Prameela Devi of ITCC. You can also get them from Directorate of Rice Research, Hyderabad and Andhra Pradesh Rice Research Institute. Dr.T.R.Sharma, Project Director of National Research Center for Plant Biotechnology present in IARI campus has worked on allele mining from Wild relatives of rice sps like Oryza officinalis , Oryza rhizomatis for resistance to Magnoporthe grisea and checked for resistance using isolates from different parts of India . So, he can spare you isolates of them.
Thanks all. I tried from all the resources but did not get positive answer. But I am trying.
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