looking for a methodology to accurately count the number of animals killed on roads each year

that killed by getting hit by vehicles

I recommend you visit http://projectsplatter.co.uk/ for information and ideas.

You could get data from some hunting bag statistics. But only for those animals which are counted for hunting bags and which really died!

We are working on this topic (a little bit), it is difficult getting accurate data: The hunters do only report the dead animals, the police only counts those accidents where any animal was hit (and only those, where the drivers want to get money from the insurance), but without species (just game animal)

there will always be a big gap: those hit but did not die, those hit and did die somewhere else, those who died on the road but not reported...

However, there is a lot of literature existing: look on the Web of Knowledge

Here are some:

Baker, P. J., Harris, S., Robertson, C. P. J., Saunders, G., & White, P. C. L. (2004). Is it possible to monitor mammal population changes from counts of road traffic casualties? An analysis using Bristol’s red foxes Vulpes vulpes as an example. Mammal Review, 34(1), 115-130.

Blanco, J. C., & Cortés, Y. (2007). Dispersal patterns, social structure and mortality of wolves living in agricultural habitats in Spain. Journal of Zoology, 273, 114-124. doi: doi:10.1111/j.1469-7998.2007.00305.x

Grilo, C., Bissonette, J. A., & Santos-Reis, M. (2009). Spatial-temporal patterns in Mediterranean carnivore road casualties: Consequences for mitigation. Biological Conservation, 142(2), 301-313. doi: 10.1016/j.biocon.2008.10.026

Gryz, J., & Krauze, D. (2008). Mortality of vertebrates on a road crossing the Biebrza Valley (NE Poland). European Journal of Wildlife Research, 54, 709-714. doi: DOI 10.1007/s10344-008-0200-0

Peris, S., Baquedano, R., Sánchez, A., & Pescador, M. (2005). Mortalidad del jabalí (sus scrofa) en carreteras de la provincia de Salamanca (NO de España): ¿Influencia de su comportamiento social? Galemys, 17(1-2), 13-23.

Roberts, C. W., Pierce, B. L., Braden, A. W., Lopez, R. R., Silvy, N. J., Frank, P. A., . . . Alpizar, J. (2006). Comparison of Camera and Road Survey Estimates for White-Tailed Deer. Journal of Wildlife Management, 70(1), 263-267.

Rodríguez-Morales, B., Díaz-Varela, E. R., & Marey-Pérez, M. F. (2013). Spatiotemporal analysis of vehicle collisions involving wild boar and roe deer in NW Spain. Accident Analysis & Prevention, 60(0), 121-133.

Thurfjell, H., Spong, G., & Ericsson, G. Factors affecting wildlife-vehicle collisions. 19.

Thanks Oliver, that what I wanted  

:-~)

another hint:

You may have a look on Wikipedia for an impression on the numbers of WVA in EU (at least on the German page).

Hi.

There are some good papers describing road casualties in owls, e.g. in Spain and Portugal. See these links:

https://sora.unm.edu/sites/default/files/journals/jrr/v022n03/p00081-p00084.pdf 

http://geofocus.rediris.es/2011/Articulo4_2011.pdf 

http://theoeco.fc.ul.pt/publications/BordadeAgua_2014_EcoMod.pdf 

http://www.ardeola.org/files/181.pdf