Check this link out: 

http://www.scbt.com/sv/datasheet-361165.html

thanx

Cyclophosphamide (CAS 50-18-0)

Physical State: Solid

Density: ~1.33 g/cm3 (Predicted)

Refractive Index: n20 D 1.51 (Predicted)

IC50: NCI-H727 proliferation: IC50 = 9.3 nM; DU-145: IC50 = 52.5 µM (human); HeLa: IC50 = 71.4 µM (human); HL-60 : IC50 = 8.79 µM (human); 3T3: IC50 = 37.6 µM (mouse)

pK Values: pKb: 2.84 (Predicted)

4 more rows

Cyclophosphamide | CAS 50-18-0 | Santa Cruz Biotech

www.scbt.com/datasheet-361165.html

Dear Haya,

Dear Colleagues,

I actually need your help to understand about which cyclophosphamide / endoxan we are speaking about.

"The" cyclophosphamide I know is a prodrug that is totally inactive in vitro (GI50 > 100 µM). It must be metabolized in vivo into the liver to become active.

Here attached are the data I downloaded from the NCI website.

But as I said, I must be wrong according to the data provided by Béatrice and Tomas, to whom I actually ask their help to understand my error.

Best regards

Robert

Dear Robert

Your note is perfectly pertinent: when we talk of cyclophosphamide in a patient we assume that it will be transformed to hydroxycyclophosphamide in the liver, which is the active drug.

But the question of Haya probably refers to cell culture concentrations, in which case the ICs are refered to the active pharmacological state of the prodrug: hydroxycyclophosphamide.

Best regard

Dear Tomas,

Cyclophosphamide is cyclophosphamide ...

Hydroxycyclophosphamide is hydroxycyclophosphamide ...

Cyclophosphamide is not hydroxycyclophosphamide ...

and I read what I read ...

Best regards

Robert

https://www.jstage.jst.go.jp/article/ahc/38/5/38_5_323/_pdf