Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

03 April 2018 4,926 4 View

Interpolation of 3D wind data (for every lon,lat,time) based on drifter positions and time (different matrix dimensions)?

Hello everyone, I am working with some meteorological data from SKIRON which I converted from grib to netcdf in order to make the necessary calculations and formatting for interpolation. I want to...

17 January 2018 6,415 3 View

What are the climatic tolerances of ants?

I can find literature discussing the temperature tolerances of individual species but not the family Formicidae as a whole. Is anyone aware of any literature discussing their climatic tolerances...

02 November 2017 5,402 4 View

What are the best procedures and techniques of teaching STEM education in the twenty first century?

I am interested in searching for the best procedures and techniques of teaching STEM to motivate and sustain students' interest in STEM particularly chemistry. p

05 September 2017 9,622 3 View

Is there a problem with using a large sample size in your data analysis after you have determined a small sample size before the study?

29 May 2017 6,595 10 View

Does injection of 18F-FDG induce an endogenous insulin response similar to normal glucose?

29 September 2016 5,189 2 View

What is the minimum soil sample size (in g) that should be used when wet sieving to examine mycorrhizal fungi spore abundance and/or diversity?

10 July 2016 7,749 11 View

Urgent: does this analysis of a repeated measure manova make sense?

background: 100 students were given an unrelated text and a related text each. they then had to answer questions on each text, say how interested they were in each text, and then evaluate how much...

30 May 2016 6,467 3 View

How can we confirm transfection of a WT gene into a cell line?

We are transfecting mutations and matched WT genes. We'll be using antibiotic selection and have generated kill curves to determine the optimal concentration of the selectable marker in the cells...

19 May 2015 4,820 8 View

What other factors can affect failure of hydrogels to form?

06 April 2015 7,411 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View