I am developing a proposal that will be testing on cytotoxicity of TfCRM107(derivative of Diphtheria Toxin) in vitro. Where or how can I acquire the immunotoxin?
In a topology optimization machine learning prediction algorithm, can I consider the OOB score of RF as the same as R^2 score. Could that be a more unbiased way of validating the model
14 February 2021 3,540 1 View
I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...
10 February 2021 180 3 View
Is there any recent work available on the performance of P2P Lending in India?
05 January 2021 5,211 3 View
I am working to improve a manuscript and I have been advised to provide a map showing where the correlation is significant. Any information or links to learn about this would be quite helpful....
26 December 2020 1,162 5 View
Water is a molecule that has a very high boiling point compared to ethanol. Why does water have such a high boiling point compared to a molecule like ethanol, and what are the...
23 November 2020 9,662 8 View
I am looking for a website of blog where i can get details about the relevant datasets used for question answering in the medical field. What i found, are not explaining in details these...
10 November 2020 7,202 3 View
Can anyone guide me to some recent studies on Impact of Microfinance in India on Poverty, Employment and Incremental Income. Many Thanks.
09 October 2020 9,962 1 View
What Economic Activities taken up with Microfinance loans can yield returns greater than 25% which is the usual cost of a micro loan in India?
09 October 2020 3,777 5 View
Many in-service teachers were not trained on ICT integration. COVID 19 has made it clear that teachers need to have been well versed with the use of ICT in teaching and learning environment. This...
27 September 2020 4,646 1 View
I will be extracting trypanosome DNA from mid-guts of tsetse flies for LAMP analysis. Kindly advice on protocol to adopt for maximum yield and benefits of Chelex method over the regular method....
23 September 2020 504 4 View
Hello everybody! I am injecting mice with Diphtheria Toxin to my mice. All the injected display neurological impairment. Did somebody experience that? Is it an effect given by the diphtheria or is...
31 December 2019 687 3 View
I am looking for a simple and low-cost method of monitoring the cholesterol content in the cell membranes. My initial idea was to stain them with cholera toxin (anti-GM1 ganglioside) to get...
10 November 2019 9,364 1 View
Can anyone share their experience on the lifetime of different tracers or dyes? I am retrogradely labeling neurons with cholera toxin (CtB) 594 for chronic in-vivo imaging. I assume the lifetime...
28 October 2019 9,546 3 View
09 October 2019 614 3 View
I am designing an experiment where I would like to cross mice expressing cre in my cell subset of interest with mice that express either cre-inducible diphtheria toxin receptor (DTR) or...
15 September 2019 7,237 1 View
My project is to prolong the half life of immunotoxin (antibody + toxin), so that they can be more potent in treating cancer. The strategy that I used is pegylation at different locations. When I...
22 January 2019 4,993 1 View
31 December 2018 9,953 1 View
Hello Scientists, I am planning to culture prostate cancer cell line and it requires addition of Cholera toxin, I am trying to find a proper way of disposal of the cell culture media and the...
07 August 2018 9,256 3 View
we aim to establish an assay using vero cells to measure the toxicity of shiga-toxin. Therefore we aim to use a method making use of the CD50. Can anyone supply me with a detailed protocol...
06 July 2018 995 3 View
We want to grow MCF10A cell lines. However, it is really difficult to get cholera toxin in Hong Kong, which is one component of the medium for MCF10A. And I checked references, it seems that...
04 May 2018 6,648 5 View