I am developing a proposal that will be testing on cytotoxicity of TfCRM107(derivative of Diphtheria Toxin) in vitro. Where or how can I acquire the immunotoxin?
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?
10 July 2024 8,763 0 View
The impact im looking for could be both positive and negative on the electric grid
18 June 2024 9,269 3 View
IT
09 June 2024 735 1 View
What are the major ethical issues to be considered while working on design and construction of electronics device in a research work.
03 June 2024 8,189 4 View
The impact of monetary policy and bank rate on the performance of deposit money banks in Nigeria
31 May 2024 7,587 1 View
The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...
08 May 2024 7,467 0 View
ls it possible to emerge the wi-fi and Li-fi in one device and regular them.
21 April 2024 9,292 0 View
The code needs to be able to work on a 28-bus system having cost as the objective function and constrain like transmission loss.
18 April 2024 3,349 1 View
I want to understand why most analyses conducted in Africa use GMM instead of other panel data estimation techniques.
16 April 2024 5,322 3 View
I am working on MCF10a cells. I started my culture by thawing a fresh vial of cells purchase in the month of February, 2024. I am culturing them in DMEM/F12 media supplemented with horse serum,...
10 March 2024 8,581 2 View
Most of the commercial antibodies available seem to target the T18 domain (aa 225-339) of the adenylate cyclase, or they do not specify which domain. I am in search of an antibody specific to the...
18 December 2023 158 0 View
Would anyone know how to answer this question? Or let me know which supplements are really needed at this stage of building the 3D skin?
01 March 2023 2,903 1 View
Hello Scientists! I’m trying to resuspend CTB-Alexa 555 from Invitrogen (C34776) as manufacturer recommend (In PBS, pH 7.4). But I found a several precipitates as the final result. Does anyone...
27 February 2023 6,675 0 View
I run a qPCR (Taqman probe) to detect shiga-toxin gene (stx1) with rotar gene Q (qiagen) thermal cycler. My primer & probe are - F - 5'-TTTGTYACTGTSACAGCWGAAGCYTTACG-3' Base - 29, GC - 44.8%,...
17 December 2022 2,981 3 View
I recently tried to induce myocarditis in BALB/c mice using the following protocol: Day 0: Subcutaneously injected 200 ug of MyHC614-229 peptide (Ac-RSLKLMATLFSTYASADR-OH) emulsified in complete...
05 December 2022 7,015 1 View
I´ve seen some toxin-antitoxin systems in bacterial chromosomal regions that are not conserved in related genomes. In addittion to their role in plasmid manteinance, are these systems reported to...
27 October 2021 9,052 1 View
With use of Mac conkey agar and broth. Please I need a hint.
08 September 2021 5,591 1 View
I am looking for a lock box to store a biotoxin in my -80 freezer? Does anyone know of any that can withstand temperatures that low? Thanks}!
02 September 2021 3,373 0 View
I am looking for the death rate of a bacterial cell due to plasmid loss in case of post-segregational killing (toxin-antitoxin system containing plasmids).
11 August 2021 4,306 1 View