When I make Chitosan microsphere(CM), I use 1% of Chitosan in 0.5% acetic acid.
After adding TPP, I harvest CM and then wash it with DI water just one time.
(At this moment, It form pellet very well)
But, when i try to do second washing, it never form pellet..(13000g & 10min centrifugation.)
I don't know what is happening.
Anybody know this, please tell me, please..