Hello everyone, I want to asses the larvicidal activity of a product on mosquito larvae, however, I don't know if the selection of the species to test the product has importance, and if there is a factor that helps to choose the species.
To test the larvicidal activity of your product on mosquito larvae you can work with Culex pipiens because is a species easy to rear and keep under laboratory conditions. If your objective is to test your product with a species of medical importance you should select Aedes aegypti.
Keep in mind that in order to assemble a reproducible and reliable bioassay you need a good sample size, with the same number of repetitions, therefore you will need a good amount of mosquito larvae available to perform the efficacy tests of your product, which means that you have to rear the mosquitos under laboratory conditions.
Further information is needed to adequately answer this. e.g. what is your larvicide (chemical/biological)? Where would your larvicide be used and why is it being considered? Is it because there is a specific nuisance/vector problem? If so, you should test it on these. Is it because there is resistance to a commonly used larvicide? If so you would need to use it against a resistant strain (whether that is Culex, Aedes, Anopheles). What is the target site of your larvicide? - this may influence what strain is used.
What kind of water body would it be used in? Are there Mansonia there? Then you might test against these.
So, there is plenty to consider but more information is needed.
Dear Nabti......your question is incomplete. Whether you want to test the lavicidal activity of the product in the lab or in the field? If in the field, the choice of species should be based on its initial effectiveness on the species of mosquito during laboratory testing. If in the lab, better to screen/test the effectiveness against 1 Anopheles species, 1 Culex species and 1 Aedes species because this will give you the spectrum of the larvicidal activity of the product. As a general rule, the choice of the species of mosquito for larvicidal activity testing depends on the availability of resources at your end.
Nabti,
If i get you well, the first point is that you must use a specific known species of your choice. This will be informed by; the chemical you are testing, its nature/presentation, its behavior in the environment etc. I think a member of the Culex pipiens complex, one that is easy to breed in the lab.
you may need to give more information for a comprehensive answer.
Dear Nabti Ismahane
You should determined species of mosquito to focus problem would be solved for larvacidal activity. Today I have been focused of Aedes aegypti larvae controlling using copepode Mesocyclops aspericornis, to test the larvicidal activity you could determined first or second instar larvae.
Munawir Sazali I think that the efficacy of a product on a species can be generalized on the other mosquito species. If I try it on a species, that does not mean that it will be effective only on the targeted species because in any way we can not try the product on all species. what do you say about that?
You question about choose the species, but you want to generalized, its different pathway to test your product..
Hello, I think a lot has been said already. You have to provide more about your question to be able to get a satisfied answer. However, as suggested above, if you are testing it in the Laboratory, you have to choose a species that is easy to breed in the Laboratory and in this case Culex pipiens Complex is very good for that.
Hi There,
The objective of your project determines which species you have to use.If that's so ,it could be the density of the local vectors(may be Anophelines, culicines,mites,ore any other arthropods) in your study area,public health or animal health importance, economic importance of the specific vector species might be the possibly good to select the larvicidal effect of your product.
Dear Nabti
Under lab conditions larvicide should be used against Anopheles, Culex and Aedes app. However, under field conditions, mosquito sp. should be decided based on the properties of larvicide, whether it dissolves quickly and completely or remain in water column for a longer time or settles down at the bottom of habitat. Ideally, under field conditions, larvicide should be tested against container breeder mosquitoes viz. Aedes aegypti,albopictus and An. stephensi etc.
The species Aedes aegypti is of medical importance because it is a vector of important arboviruses such as dengue, zika and chikungunya. If you are going to start larvicidal studies, it is a good species to start your work.
I agree that the objectives of the study and the Mosquito species in question. Of importance is the mode of action of the larvicide and it's behaviour in water. Does it float on the surface the Mosquito app with larvae that rest at the surface would come into contact with the larvicide. If the larvicide spreads across the water both surface and deep, then a large number of spp can be used in the study.
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