Do you think we can monitoring special material in plants via live organism as a bioindicator?

for example some bee can recognize some gums on plants and can know them from other kinds of gum

09 October 2016 2,736 7 View

Do you think dodder substances are changed according to it's host?

some traditional material are obtained from dodder, orbanch, etc. are these things change by changing host?

07 August 2016 3,653 0 View

Do you know how long a new variety has to preserve it's ability?

we know each variety lose some characters during years. some of these traits are very important for us, such as yield, essence, resistance to pests, etc. how long are these characters fix?

07 August 2016 4,528 6 View

Can parasites can change quantity of plant substances?

07 August 2016 4,887 0 View

Do you know anything about leech and leeching?

i need scientific and modern method.

31 December 2015 8,809 3 View

Are there any standard methods for producing or marketing of leech?

31 December 2015 7,998 3 View

Is there any validated information about stones effect on human health?

31 December 2015 2,293 3 View

How many plants are there in the world? do you know valuable data?

"250000-300000 species" is correct?and what about cultivars/varieties?

10 November 2015 9,101 2 View

What is Orthodox medicine?

Herbal and orthodox medicine

10 November 2015 2,233 3 View

Is the color of essential oil a good guide?

can we provide a descriptor for essential oils color in one family/genus?

08 September 2015 5,700 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View