Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

how to calculate GO enrichment analysis for all predicted complexes ?

I am developing an algorithm to predict protein complex and I want to measure the GO enrichment for all the predicted complexes which tool should I use to do such analysis for all predicted...

30 March 2020 155 3 View

How to teach Interpretation (Oral Translation) to a class of 40 students?

What are some of the strategies/activities you have found effective in teaching interpretation skill to students in your classrooms? How to teach Interpretation (Oral Translation) to a class of 40...

26 July 2018 2,388 6 View

Do you think it is accurate to assume that Chinese, Mandarin, is the most difficult language to lean comparing to all the world languages?

I have heard a lot of people say that Chinese is the most difficult language to learn. What are your views? Do you believe it is accurate to assume that Chinese/Mandarin really is the most...

01 July 2018 3,579 42 View

In your opinion, what is the single most important quality of a good students?

In your opinion, what is the single most important quality of a good students?

19 June 2018 3,242 92 View

How to prepare GROMACS ligand topololgy ?

Hi, I used PRODRG ( http://davapc1.bioch.dundee.ac.uk/cgi-bin/prodrg ) to prepare topology of my ligand but recently i find out that the charges often don't come out right so we have to modify...

17 February 2018 7,973 9 View

Which is better, Comsol or Fluent for turbulent flow modeling in Copper electrowinning in the presence of magnetic field (magnetoelectrolysis) ?

This problem is a multiphysic problem and a turbulent flow of electrolyte with ionic species flow in the electrowinning cell continuously. under the electric field reaction on cathode and anode...

02 February 2018 9,019 4 View

Immunoflorescence of blood cells

I need to do immunofluorescence on tumor section to track different subtype of blood cells accumulated in tumor. Which markers do you recommend? I was thinking about CD11b (for all myeloid cells...

21 March 2016 5,751 3 View

What do you specifically look for when choosing a university to pursue your post-graduate studies?

Choosing the right university to pursue post-graduate is extremely important. What specific criteria to keep in mind when choosing a university to pursue post-graduate studies?

01 January 1970 4,619 13 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View