Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

how to calculate GO enrichment analysis for all predicted complexes ?

I am developing an algorithm to predict protein complex and I want to measure the GO enrichment for all the predicted complexes which tool should I use to do such analysis for all predicted...

30 March 2020 155 3 View

How to teach Interpretation (Oral Translation) to a class of 40 students?

What are some of the strategies/activities you have found effective in teaching interpretation skill to students in your classrooms? How to teach Interpretation (Oral Translation) to a class of 40...

26 July 2018 2,388 6 View

Do you think it is accurate to assume that Chinese, Mandarin, is the most difficult language to lean comparing to all the world languages?

I have heard a lot of people say that Chinese is the most difficult language to learn. What are your views? Do you believe it is accurate to assume that Chinese/Mandarin really is the most...

01 July 2018 3,579 42 View

In your opinion, what is the single most important quality of a good students?

In your opinion, what is the single most important quality of a good students?

19 June 2018 3,242 92 View

How to prepare GROMACS ligand topololgy ?

Hi, I used PRODRG ( http://davapc1.bioch.dundee.ac.uk/cgi-bin/prodrg ) to prepare topology of my ligand but recently i find out that the charges often don't come out right so we have to modify...

17 February 2018 7,973 9 View

Which is better, Comsol or Fluent for turbulent flow modeling in Copper electrowinning in the presence of magnetic field (magnetoelectrolysis) ?

This problem is a multiphysic problem and a turbulent flow of electrolyte with ionic species flow in the electrowinning cell continuously. under the electric field reaction on cathode and anode...

02 February 2018 9,019 4 View

Immunoflorescence of blood cells

I need to do immunofluorescence on tumor section to track different subtype of blood cells accumulated in tumor. Which markers do you recommend? I was thinking about CD11b (for all myeloid cells...

21 March 2016 5,751 3 View

What do you specifically look for when choosing a university to pursue your post-graduate studies?

Choosing the right university to pursue post-graduate is extremely important. What specific criteria to keep in mind when choosing a university to pursue post-graduate studies?

01 January 1970 4,619 13 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to change medium of a protein or peptide mixture?

Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...

27 January 2021 4,055 4 View

Rhodanese enzyme assay using salt potassium ferricyanide?

I wanted to perform rhodanese enzyme assay using salt potassium ferricyanide (K3Fe(CN)6). The assay is based on the products formed in the reaction but I can't get the reaction i.e what product...

26 January 2021 8,044 4 View