Hi. I am pretty new on the field. I just joined a lab and started my reading.

I intended to work with yeasts as a potential host for my project. I wanted to integrate a certain pathway that involve about 7-8 enzymes. Some of the enzymes are produced endogenously, while others need to be introduced heterogeneously from other species.

So I was planning to do Gibson assembly to assemble a "pathway casette" that contains genes expressing 4-5 enzymes. But I am not sure whether I can clone it to my vector they way how to do cloning to bacteria.

So I have been reading some paper and came across these terms when working on yeasts: transposition, chromosomal integration, Ty7 element, 2u plasmid. So far when I have read the cloning process in bacteria, it only involves restriction enzymes, the correct vector, promoter, gene insert, and selectable marker genes. But when I read about yeast transformation, I was confused with all these terms.

I would appreciate if somebody could enlighten me on this.

It seems to me in yeasts we can either do chromosomal integration or normal gene-to-vector assembly. When to use both? Are they just different techniques, or each serve different purpose? Thank you all in advance. 

Regards.

Ino