Kemas Aurino

3 Questions 10 Answers 0 Followers

Questions related from Kemas Aurino

When to integrate our gene casette to chromosome and when to just assemble it into our vector?

Hi. I am pretty new on the field. I just joined a lab and started my reading. I intended to work with yeasts as a potential host for my project. I wanted to integrate a certain pathway that...

07 July 2017 1,829 0 View

Any suggested vector for Saccharomyces boulardii metabolic engineering?

I always have been curious how scientists build a vector. I intended to integrate certain pathway (with 5-7 enzymes involved) into S. boulardii., but need suggestion on what vector to use,...

07 July 2017 5,841 3 View

To use cDNA or the whole native gene sequence?

Hi, I am aware that yeasts are eukaryotes and have the ability to do splicing such as in other eukaryotes. But I wonder, if let say, I want to express heterologous gene A in yeast, if I enter the...

07 July 2017 9,586 4 View