When should I inject CNO for DREADDs system before social behavior exp. ??

Abdelrahman Alkahwaji @Abdelrahman-Alkahwaji

11 August 2017 1 3K Report

I want to do a circuit study on social behavior test. I will use inhibitory DREADD to investigate the role of specific brain regions in this particular behavior. I wish to inject the CNO intraperitoneal to inhibit this brain circuit before the experiment. Could you tell me when should I inject the CNO in mice? before which time should I inject the CNO? before 10 or 20 min?

Jai Ghosh

Usually between 10 and 20 minutes. Never before 10 minutes.

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