dear Moaed,

When sowing has been done in soil what we measure is 'emergence' not 'germination'. Once plumule has emerged from soil, it is considered that emergence has taken place.

Thank you Kumar, any reference if you have?

Hi dear

You can use the scan method. Therefore, the plant needs to be planted in a glass vase, then use a radioactive marker and scan the root after use.

Or have replicates and you dig up the seeds at varying intervals

Thanks Mahmoud and Andrew

When cotyledon leaves are expanded

thank you cosme

With the rupture of seed coat and emergence of plumule, a seed can be considered as germinated.

Thank you Anup

The emergence of the embryonic axis (usually the radicle) from the seed coat considered as seed germination.

Thank you Somnath

Dear Moaed,

If you are assessing the effect of soil on seed germination, so you should delicately dig and put the seed back quickly.

Hi Moaed Almeselmani,

2. An alternate way is soaking the seeds in water or moist filter paper for the necessary time to allow the germination and measure the radicle length at a definite time interval using a stereo microscope.

pls find the attachment-related to radicle emergence test

Technical Report Application of the radicle emergence test to radish (Raphanu...

Article Seed vigor classification using analysis of mean radicle eme...

Regards

Nagendra Gowda