When running cytochrome b pcr for Mastomys natalensis, is it compulsory to have only positive results?

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Krisztián Laczi

If the genome contains the gene and primers can hybridize then yes.

If you get negative results you should check primer sequences, optimise PCR parameters such as annealing temperature. You may supplement buffer with DMSO and/or magnesium if necessary.

Debaprasad Koner

Dear Bongo,

There is no such gene specific universal rule to get always positive result. It is solely depends on the primers and other reaction parameters. If you are not getting the positive result, check the parameters as Laczi mentioned. It would be easier to troubleshoot, if you share more information about your experiment.

Hope for the best.

G.N. Bongo

Thanks a lot for your answers... I appreciate