In our one experiment, culture characters of one fungal isolate was found similar to Fusarium species. However, in absence of macroconidia it is difficult to screen its species. We had used several standard (PDA, MEA, OMA, SDA, CMC, etc.) and plant enriched culture media and incubated in different combinations of temperature, pH, moisture, light and time but fungus synthesizes only microconidia.
dear Rajendra ...may be strain not fusarium ...how diagnostic as fusarium ...
Thanks Neeran and Daniel for your suggestions,
I had already used CLA for the study of culture characters of fungal isolate.
Dear Readers,
We had isolated this fungal pathogen from maize caused by stalk rot disease. Microscopic observations including shape and size of microconidia (except macroconidia which was not synthesized) and culture characters were found similar with Fusarium sp.
Dear prasad try and see Mung bean broth incubate for 7day you may observe Macroconidia and also try wheat bran 1g boiled in 100ml water
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