I am currently doing total phenolic content using the Folin-Ciocalteu method for my project on olive fermentation, but encounter a problem as my extract was too thick that I struggled to pipette it. So can I centrifuge my sample and will the results still be accurate
If you centrifuge or not, you could still get the results, only with differences from the interpretation of concentrations. You can test straight without centrifuging. or if you want to use a different solvent to extract than the initial one, then centrifuge should be carried out followed by complete evaporation.
Please take a look at the following link containing some documents for references, it might help.
https://www.researchgate.net/post/How_do_I_extract_polyphenols_using_folin_ciocalteu_method
Yes; I thing that you can centrifuge your sample and your results will still be accurate . I allways centrifuge my sample at 6000g for 15 min when doing phenolic content using the Folin-Ciocalteu method, and the results are OK.
Andrew Mtewa, Thank for the link: I leaned that; it is not really correct to talk of "Total phenolic" content dueto the interference from ascorbic acid, proteins.......
I think you must centrigugate your sample, your results won't have repeatability if you have suspensions. Folin Ciocalteu assay is a very bad test in the sense that it gives a lot of false positives, for instances reducing sugars will had to the value you find and they are phenolics.
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