What's the most representative method for specimen sampling from the human's breast tissue in order to study its microbiota?

10 October 2017 1 7K Report

I'm working on a sampling protocol in order to study the total microbiota of the mammary gland tissue in other species than humans (notably in food animals). I'm interested about current methods in humans (specially sample treatment after collection) that could be applyed in other species.

Christine Ann Edwards

I would recommend the methodology suggested in the following article although other researchers don't use iodine but use other antiseptic washes to remove contamination from the skin which may also be very good.

The human milk microbiome changes over lactation and is shaped by maternal weight and mode of delivery1,2,3,4 Raul Cabrera-Rubio, M Carmen Collado, Kirsi Laitinen, Seppo Salminen, Erika Isolauri, and Alex MiraAm J Clin Nutr September 2012 vol. 96 no. 3 544-551

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