Size-exclusion chromatography for the isolation of EVs?

Dear all, I am currently doing research on extracellular vesicles (EVs). I'd like to improve the isolation method by trying out size-exclusion chromatography (SEC) compared to serial...

07 September 2020 4,412 5 View

You can support me in a study on COVID-19?

Hello, I am a psychological researcher. We are conducting an investigation on COVID-19 and I hope to have your support....

17 July 2020 8,402 13 View

Which is the optimal optical density for toxical recombinant protein expression in E.coli BL21 (AI)?

Hi everyone. I am trying to express and purify a recombinant antimicrobial peptide using as a host cell E. coli BL21 (AI) (employing L-arabinose and IPTG). Because this peptide can be toxic to...

19 February 2020 4,888 4 View

How reliable are third-party pollsters such as SurveyMonkey, Qualtrics and QuestionPro for your thesis?

I do understand they are being used in consumer or market research. But what about if you engage them for your thesis? Are they reliable? Because of time constraints and for convenience, some...

13 December 2019 493 3 View

Is it reasonable to perform Free Energy Landscape (FEL) anallysis on molecular dynamics simulations of 50ns?

Hello everyone, I'm having an issue with a review of a paper. I performed 50ns MD simulations on docking poses to know if the predicted poses were stable. Among the literature doing similar...

24 October 2019 4,608 5 View

Can LiCl precipitation be used in purifying low RNA concentration (30ug/ml) ?

RNA which has been extracted from blood collected in Heparin tubes are not showing any amplification after cDNA synthesis, in comparison to Blood of same patient collected in ETDA tubes. Any...

25 September 2019 9,026 2 View

No title

07 August 2019 2,592 2 View

No title

04 August 2019 5,642 2 View

I have received a ginger extract, and it is written concentration 25%, and it is one liter. so what does it mean?

My research is to study the ginger effect on breast cancer cells. so how to know the best concentration to treat the cells with it to study the effect. Note that i will treat it with different...

16 February 2019 4,419 3 View

Is there a survey I can use that asks people to rate behaviors?

I am currently looking for an existing survey that will help me ask students how they view behavior. I am thinking that I can ask them to rate how appropriate a situation is (e.g. crying in...

22 October 2018 9,145 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View