Hi all, I found two groups of genes showed differential CHG methylation in gene body regions, I am wondering the potential biological significance of The difference. Can anyone give some information or references. Many thanks in advance.
I am curious about the relationship between the hull size and grain size. Usually big hulls give big grains, which suggests that hull size is a determinator of grain size. My question would be the...
10 November 2015 1,379 1 View
I made a fused gene using gene 1 promoter to drive gene 2 expression. Because 3' of the gene 1 promoter is GC only, it was difficult to design primer for promoter amplification. To avoid this...
09 October 2015 1,096 3 View
I am planing to conduct a rice endosperm ChIP-seq experiment. Our interest is for the early developmental stage (no more than 96h after fertilization), therefore, endosperm tissue collection is...
06 July 2014 7,834 3 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View