I am planing to conduct a rice endosperm ChIP-seq experiment. Our interest is for the early developmental stage (no more than 96h after fertilization), therefore, endosperm tissue collection is tough for us considering the samll rice endosperm size. For all the ChIP-seq protocols I read, they always use more than 1g tissue for the experiment. I would like to know what's the minimum tissue amount you guys have used for a successful ChIP-seq or ChIP-PCR.Thanks for your information in advance.
It all comes down to number of cells within the given tissue and how efficiently you will be able to lyze the cells to release chromatin. I have worked with tissues where we first dissociate the tissue using enzymes and mechanical forces and then sort out specific populations by FACS. Using antibodies against histones you can get nice enrichments with 100 000 cells. Based on this I would assume that you should be able to get away with way less than 1g.
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