what's the difference between scaling two images based on scale factor (microns per pixel in each image) and scaling the images randomly (e.g bring two images to the same size regardless the scale factor)?
I am not sure what the scale factor is. it required to know the distance and focal length etc... information.
Maintaining the aspect ratio vs brute force (random)
Hi all, I'm interested in X-y location of a point on an image. If I interpolate the image to be 1000 by 1000 pixels, then downsample it to 200 *200 pixels. Then measure X-Y location of the point....
06 July 2019 3,026 8 View
Hi All, If the metric ( cost function) is very sensitive to the step size of the optimizer and the number of iterations, what does it mean?!. Does that mean the metric has a limit then the...
04 May 2019 6,080 4 View
if A (fixed image or reference image) , B ( moving image),and C is the resulted image after the registration. What should be C-B? If C-B= 0, does that mean good registration ? Thanks,
02 March 2019 7,458 3 View
Why do we need to down sample the histology image when we try the registration to a medical image?. one reason that I know is to reduce the noise. Also, to help the similarity metrics to find a...
01 February 2019 767 9 View
What are the limitations of using mutual information as similirty measure of the registeration of two different contrast machnisims?
09 October 2018 6,509 2 View
I'm facing a difficulty in evaluating the registration accuracy by using Elastix, since I'm using an artificial images. I would like to have an advice on how to evaluate the accuracy of the...
08 September 2018 8,598 5 View
Hi All, I'm using Elastix software to do the registration between a histology image and MRI? I have some questions: 1. what do you think the most important parameters that could affect the...
07 August 2018 8,636 1 View
I made a registration between fixed and moving image using affine transform and differences metric. I subtracted the result image from the fixed image to evaluate the registration. As you see in...
07 August 2018 5,658 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Anomaly detection in scanned image data set
18 July 2024 3,578 3 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View