If C-B= 0, does that mean good registration ?

More Wadha moh Alyami's questions See All

What's the image resizing impact on the accurate x-y location of a point?

Hi all, I'm interested in X-y location of a point on an image. If I interpolate the image to be 1000 by 1000 pixels, then downsample it to 200 *200 pixels. Then measure X-Y location of the point....

06 July 2019 3,026 8 View

Why does some metric (cost function) require very short step size of the optimizer to give good registration results?!?

Hi All, If the metric ( cost function) is very sensitive to the step size of the optimizer and the number of iterations, what does it mean?!. Does that mean the metric has a limit then the...

04 May 2019 6,080 4 View

Why do we need to down-sample a histology image ?

Why do we need to down sample the histology image when we try the registration to a medical image?. one reason that I know is to reduce the noise. Also, to help the similarity metrics to find a...

01 February 2019 767 9 View

What's the difference between scaling images based on scale factor and randomly?

what's the difference between scaling two images based on scale factor (microns per pixel in each image) and scaling the images randomly (e.g bring two images to the same size regardless the scale...

10 November 2018 4,960 2 View

Limitations of the mutual information?

What are the limitations of using mutual information as similirty measure of the registeration of two different contrast machnisims?

09 October 2018 6,509 2 View

Is there anyone expert with using Elastix for image registration ?

I'm facing a difficulty in evaluating the registration accuracy by using Elastix, since I'm using an artificial images. I would like to have an advice on how to evaluate the accuracy of the...

08 September 2018 8,598 5 View

Questions in images registration ?

Hi All, I'm using Elastix software to do the registration between a histology image and MRI? I have some questions: 1. what do you think the most important parameters that could affect the...

07 August 2018 8,636 1 View

What could cause the symmetric shape that resulted after the subtraction of registed moving image from the fixed image?

I made a registration between fixed and moving image using affine transform and differences metric. I subtracted the result image from the fixed image to evaluate the registration. As you see in...

07 August 2018 5,658 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View