To be honest I have no experience in handling RNA at all.
I will have whole blood and have to isolate total RNA from it and carry out expression microarray.
Since the RNA isolation is not carried out immediately, my question is, how to store the blood with red cells removed so that the RNA is not degraded?
And what would the subsequent protocol/kits be to isolate total RNA from that storage method? The RNA is used for expression microarray.
Hi, you can check this topic it already has a lot of answers.
https://www.researchgate.net/post/What_is_the_best_protocol_for_RNA_isolation_from_whole_blood_TRIzol_or_RNA_Isolation_kits
To store it you will either need to freeze immediately (never tried, so don't know if it works well or not) or use buffers containing specific stabilization solutions (like RNALater)
Yes I did find that topic as well. Someone suggested to use Trizol+ Qiagen kits to isolate total RNA, but I just don't know how should I put the product from Trizol to the Qiagen kits from downstream process.
Does anyone knows? and which kit from qiagen should I use after Trizol extraction?Thanks
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