What's the best way to determine Titin isoform ratio?

09 March 2017 1 5K Report

I haven't been able to find two separate western antibodies for it, and I was wondering what other people have used?

Dear Jeremiah Ockunzzi: Attached file provides deep insight into the biochemical analysis . Hope this probably be your required answer

Badges
Science topic

More Jeremiah Ockunzzi's questions See All

What do old testament sanctuary system mean?

What is actually to discuss in old testament sanctuary system is, It origin, symbol and their meaning, significant rules, guidelines for operation and salvific implications.

11 June 2024 9,421 4 View

Are the synthesized nanocatalysts able to maximize sustainability in biodiesel production while reducing their negative effects on the environment?

This research question draws upon various areas of expertise such as biodiesel production, heterogeneous catalysts, optimization, and life cycle analysis. It presents opportunities for...

29 April 2024 2,453 1 View

How to improve Sanger Sequencing?

Dear all, I am having problems with my Sanger sequencing results, the chromatograms are not looking good (please refer to the attachments for images and ab1 file). May I ask if anyone knows what...

26 December 2023 8,023 21 View

What's a fungal strain?

Hi, I am new to mycology, may I ask what's a fungal strain? how to identify a different fungal strain from the other? do i need to produce them in order to describe a species properly and how may...

16 December 2023 523 12 View

Looking for research on the topic. Influence on emerging management issues in effective implentation of Competency Based curriculum ?

May I get some recommendations and Literature?

04 October 2023 9,126 1 View

Is there a video suvaillance dataset that shows traffic accidents?

I need a dataset of traffic cams that shows accidents and the moments before they happened so I can get a behvioural analysis on patterns that results in accidents

23 September 2023 426 4 View

Would you please help me with a business plan?

urgently need a business plan for starting a finance company with a minimum capital of K1000.

22 August 2023 3,251 10 View

Is there a simulation of NB-IoT on HTTP that i can watch to see how this works?

I am interested in NB-IoT technology, I looking for a model of NB-IoT based on HTTP. Looking for a link for the simulation of sensors connecting to a database and users accessing it from a web...

19 July 2023 4,717 1 View

Is there a simulation of NB-IoT on HTTP that i can watch to see how this works?

I am interested in NB-IoT technology, I looking for a model of NB-IoT based on HTTP. Looking for a link for the simulation of sensors connecting to a database and users accessing it from a web...

12 July 2023 9,978 1 View

Can anyone help with optimizing script for MORGAN v3.4 (PANGAEA) parametric linkage?

So my partner is struggling with getting the MORGAN v3.4 (PANGAEA program) parametric linkage to generate appropriate results, there are absurd LOD scores even for the "dummy" data (where they...

25 February 2023 8,925 0 View

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Do you know best mines of western part of Afghanistan?

I want to know more about Mn deposits in west of Afghanistan.

07 August 2024 3,427 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View