Hi i would like to analyse a synthesized nanoparticle using BET, SEM and TEM? Can anybody assist?

Hi i am intending to characterize some nanoparticles, i there anybody who can assist?

03 March 2018 9,337 8 View

Do we have related studies on student-centred approach to a large class setting?

many studies on learner-centred education are subject specific to assess the impact on performance. however, i am looking for available research that links practicality of teaching a large class...

01 November 2017 7,486 4 View

ATP Solution in Basic pH?

Hello, I have been trying to make a buffer that contains 2.5mM ATP in a basic solution. However, whenever I attempt to make the buffer it becomes cloudy, and some sort of precipitate settles at...

01 October 2017 9,430 1 View

Does anyone know of a way to reuse or wash Zip Tips?

15 January 2015 4,272 4 View

How do I handle waste water samples for maximum results in a research involving mercury?

Most of the techniques mentioned will require Hg to be as Hg++. I have to digest my samples completely before using any of these techniques.

09 May 2014 503 7 View

Which is the best method of analysis of mercury in wastewater?

I am planning to do a research on the levels of mercury in waste water; mercury is known to be a persistent, bio-accumulative toxin, indicating that its toxicity does not diminish through...

07 May 2014 4,068 38 View

How do we calculate the standard deviation of a population in research?

In my research on the "Analysis of the levels of arsenic in home-made brews, spirits, in water and raw materials using HGAAS in Nairobi county" I found out that the concentrations of arsenic in...

16 January 2014 4,207 0 View

Why is the Concentration on Nitrate always higher than that of Nitrite in Solutions?

Nitrate is more stable and can be transformed into nitrite. Nitrite levels in most water supplies are very low.

27 December 2013 3,203 4 View

Why was the MCL for arsenic in water revised from 10 to 50 ug/l?

On January 22, 2001 EPA adopted a new standard for arsenic in drinking water at 10 parts per billion (ppb), replacing the old standard of 50 ppb. The rule became effective on February 22, 2002....

25 December 2013 5,846 3 View

Is Anti-Beta-Amyloid (anti-Ab) antibodies used as a treatment for Alzhiemer's disease or is it used as more of a stain in immunohistochemistry (IHC)?

I'm pretty new to this field. My impression of anti-A beta (e.g. 6E10) antibodies are that they are used more as a stain/marker for either APP, AB40 or AB42 in IHC (Aho et al 2010:...

31 July 2013 846 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View