Usually we detect the cytokines production by ELISA. But sometimes it's not sensitive enough. So we decided to try to look at their genes expression as well.
We are looking for their best primers. The reference for them would be great for us both for human and mouse.
Current understanding of the biocompatibility of gold nanoparticles
is controversial. We studied the acute (1 day) and sub-chronic (5
days) effects of gold nanoparticles (10 and 50 nm in diameter) on
expression of interleukin-1 beta (IL-1b), IL-6 and tumor necrosis
factor alpha (TNF-α) in rat liver. Real-time PCR analysis showed
that gold nanoparticles of both sizes significantly increased cytokine
gene expression on day 1; this had subsided by day 5. The 50-nm gold
nanoparticle produced more severe inflammation than the smaller
gold nanoparticle.
This is a very good place to start for primers:
https://pga.mgh.harvard.edu/primerbank/
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