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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate
26 July 2024 7,813 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on...
14 July 2024 5,634 2 View
Dear all, I have a difference in the weight ratios between EDS mapping. What should I rely on? Is there a way to solve this problem?
12 July 2024 305 1 View
