Dear everyone,

I'm analyzing the diversity of species in the diet of a wild animals (detected in feces). I would like to compare the diversity by gender, site and month. To do so, I will use alpha and beta diversity. However, I am puzzled while preparing the data in R. What I should define as a sample here, should be the site, the animal, the month-site-gender?

It's my first diversity analysis and it's not clear to me. I am following: Statistical Analysis of Microbiome Data with R and https://grunwaldlab.github.io/analysis_of_microbiome_community_data_in_r/index.html

Have a pleasant day,

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