Following the series, the next puzzle is about equipment bottlenecks. Sometimes, the biggest challenge in scaling up protein purification isn’t the protein - it’s the gear. When you’re purifying at prep scale, even well-optimized protocols can run into hardware ceilings that force compromises on purity, yield, or run time. I’m curious to know about your experience - i) What’s your biggest equipment constraint when purifying proteins in bulk? ii) Is it column size, pump pressure, concentrating membrane unit, automation limits or something completely different? Let’s share workarounds and creative fixes; maybe we’ll uncover a few “wish list” upgrades for the perfect large-scale purification setup.

LinkedIn discussion link : https://www.linkedin.com/feed/update/urn:li:activity:7362226322498076673/