I am using Countess II FL automated cell counter to count COS1 & LNCaP cells. I can see that the counter is detecting some objects as cells, which are clearly not cells (can be cell debris- too small or too large, abnormal shape, some have crystalline look). I’m trying to solve the issue by optimizing some parameters (in Countess II) like cell size, brightness, cell circularity and focus of the instrument and also spinning down trypan blue before use. I have a general idea that the average cell size/diameter would be around 20µm for both COS1 and LNCaP cells. But what should be a good range (like 10-40µm) to include almost all the cells and exclude other objects from the count?
I couldn’t find any relevant information on ATCC websites!
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