Hi Augusto, I believe that Aggrastat (Tirofiban) is an inhibitor of platelet integrin aIIbB3.
I found a paper comparing Echistatin, Roxifiban, Orbofiban, Tirofiban and Eptifibatide.
Hope this helps.
http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2007.02351.x/full
Dear Augusto,
from your question I reckon you are not trying to do this in vivo...
Therefore, there will probably be no available data answering this question. However, maybe you can get some clues out of the summary of product characteristics issued by the manufacturer ("Fachinformation").
Another way to find out would be to use a test like Multiplate and test for the concentrations needed. However, this would probably mean you need to do some work first...
Can you specify your needs a little more?
http://www.fachinfo.de/suche/fi/003444
Dear Colleagues,
first of all I would like to say thank you for taking your time to answer my question.
A bit more of information about my experiments:
I am isolating platelets from human donors and testing the platelet aggregation using an aggregometer. I would like to abolish the platelet aggregation collagen-induced inhibiting the integrin aIIbB3 . Basically I will put the washed platelets in cuvetes, preincubated with the Aggrastat during 2 minutes and after that to add collagen I (10µg/ml).
I checked some manuscripts and for the in vivo experiments the concentration of Aggrastat is around 1,25µg/ml. However, I tried to repeat this experiments using the same concentration and I could not get an complete platelet aggregation inhibition collagen-induced.
That´s why I am writing the question to get some suggestions before to try the experiment again. Of course it is in my plan to do a titration curve using Aggrastat. However, I wanted to ask here and get more information before to try it again spending time and money.
Best Regards,
Augusto
Hi Augusto, I found this paper looking at gpIIbB3 antagonists:
http://atvb.ahajournals.org/content/27/3/E9.full
However, I cant find the mechanism by which Tirofiban (Aggrastat) inhibits gpIIbB3. It is not an RGD containing small molecule like other gpIIbB3 inhibitors.
Do you know the mechanism of how Tirofiban inhibits gpIIbB3?
Plus, I found this paper saying that Eptifibatide and 7E3, but Not Tirofiban, Inhibit αvβ3 Integrins on smooth muscle cells
http://circ.ahajournals.org/content/104/5/582.full
Eptifibatide is an RGD mimetic peptide and 7E3 is an anti-B3 monoclonal.
I'm bringing this up because I found some papers showing that platelets express avB3
http://medtextfree.wordpress.com/2012/01/23/chapter-111-platelet-morphology-biochemistry-and-function/
http://th.schattauer.de/en/contents/archive/issue/902/manuscript/4944.html
Albeit that avB3 expression on platelets is much lower than gpIIaB3, it could have an effect in your assay.
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