Augusto Martins Lima

5 Questions 5 Answers 0 Followers

Questions related from Augusto Martins Lima

Platelet-rich plasma vs washed platelets?

Testing the ability of drugs to modulate platelet function is generally done using washed platelets (WP). I understand that most of the publications use WP to avoid any interference of the plasma...

06 May 2017 5,474 6 View

How to fragment frozen human tissue without thawing it?

Dear colleagues, I have a human aorta frozen at -80°C and I want to fragment the sample in small pieces without thawing it. Afterwards, I want to perform PCR and immunostaining. How can I do it?...

09 September 2016 3,144 2 View

Is there any GPVI inhibitor currently being used in humans?

GPVI is a glycoprotein receptor for collagen which is expressed in platelets. I would like to know if there is any GPVI inhibitor currently being used in humans? If not, is there any commercially...

09 September 2016 2,287 3 View

Does anyone have a nice protocol to immortalize human primary fibroblast and CAF?

Does anyone have a nice protocol to immortalize human primary fibroblast and CAF (cancer associated fibroblast)? I am using commercial Htert lentivirus and I would like to know the MOI to...

19 July 2016 4,465 1 View

What´s the minimum concentration of Aggrastat (alpha2b beta3 integrin) necessary to inhibit platelet washed human aggregation induced by collagen?

Aggrastat is an alpha2b beta 1 integrin inhibitor

04 November 2014 1,016 4 View