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Questions related from Augusto Martins Lima
Several studies have shown that PPP decreases the signal of light transmission aggregometry and this effect is not related, either by the volume expansion or by ADP-released during the...
28 March 2018 240 3 View
Using an aggregometer, I am incubating washed platelets with ristocetin and von Willebrand factor. The incubation results in an increase of transmitted light intensity in my samples. Since I am...
19 June 2017 2,175 11 View
Testing the ability of drugs to modulate platelet function is generally done using washed platelets (WP). I understand that most of the publications use WP to avoid any interference of the plasma...
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I would like to know if I can induce platelet aggregation on PRP using only fibrinogen. If yes, what is the concentration of fibrinogen I should use?
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Dear colleagues, I have a human aorta frozen at -80°C and I want to fragment the sample in small pieces without thawing it. Afterwards, I want to perform PCR and immunostaining. How can I do it?...
09 September 2016 3,154 2 View
GPVI is a glycoprotein receptor for collagen which is expressed in platelets. I would like to know if there is any GPVI inhibitor currently being used in humans? If not, is there any commercially...
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Does anyone have a nice protocol to immortalize human primary fibroblast and CAF (cancer associated fibroblast)? I am using commercial Htert lentivirus and I would like to know the MOI to...
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Aggrastat is an alpha2b beta 1 integrin inhibitor
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