How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,568 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,601 6 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 1,584 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 9,527 11 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

01 October 2019 8,682 4 View

What is the minimum sample size for factor analysis?

I will be doing ryff's psychological well-being scale for psychometric properties. What would be the minimum sample size for factor analysis using spss? I read Slovin's formula is not reliable?

26 April 2017 601 7 View

Could alternate form reliability be administered simultaneously?

I will be doing ryff's psychological well being scale's psychometric properties. Could I administer alternate form reliability simultaneously? (42-item and 18-item)

25 April 2017 8,184 3 View

IS Confrimatory Factor Analysis CFA and WLS enough for psychometric evaluation?

Im going to do a psychometric evaluation on ryff's scale using nurses as my sample. Would CFA and WLS suffice? Thank you!

13 April 2017 4,938 3 View

Can Zung's Depression Scale be administered by a student?

I am currently doing my psychometric evaluation on ryff's scale and would like to use Zung's scale for my divergent validity. I am an undergrad student. Can I administer it?

09 April 2017 510 5 View

What would be a good discriminant validity for Ryff's PWB?

04 April 2017 5,633 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View