I want to isolate a protein through anion exchange chromatography using Q sepharose. The theoretical PI of protein is 9.02, Molecular weight is 139312.18, Number of amino acids is 1290, Total number of negatively charged residues (Asp + Glu) is 95 and Total number of positively charged residues (Arg + Lys) is 108. I'm just wondering for suitable buffer conditions like what should be the pH and strength of protein storage buffer, column packing buffer and elution buffer.

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