What would be the recommended concentration of Lipofectamine RNAiMAX Reagent for transfection of 200 pmol of miRNA mimic in 24-well dish?

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Alexis Varin

The manufacturer recommends 10 pmol of RNA for 3µL of Lipofectamine RNAiMax in a 24-well I'm not sure how it scales for 200 pmol, 60 µL seems way too much.

https://tools.lifetechnologies.com/content/sfs/manuals/Lipofectamine_RNAiMAX_Reag_protocol.pdf

Vipul Bhatia

Thank you Alexis. That's the problem I am also facing. That's why I was asking someone who would have used lipofectamine to transfect large quantity of mimic.

Alejandra Romero

How much time do you transfer HUVEC with RNAiMAX ?