What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

New problem Statement on Solar Energy?

Dear All, I want to know the new research topic on Solar Energy for my PhD. work. Please let me know.

13 January 2020 4,388 1 View

How to fix the low pressure problem in Ion Chromatography?

I am using Thermo Scientific Dionex ICS-1100. Even after purging(with N2) the tubes connected with Eluent Reservoir and Eluent Valve; Eluent Valve to Pump Heads & changing the eluent I am...

01 October 2019 8,954 2 View

What is the appropriate way / index of expressing dissimilarity of elements in a large sample collection?

Ex. I have taken a sample of x papers. Total number of contributing authors in these x papers are N authors. However, some authors have contributed in more than one papers in the sample. Say the...

03 July 2019 9,941 1 View

What is the lowest concentration you should take for determination of solubility of compounds by LC-MS method?

I have to determine solubility of LC-MS of completely new compound, however, the sample provided is very low. So, I would like to know what is the safe concentration to start.

07 February 2019 6,945 3 View

How to determine the connectivity of the pores through BET Isotherm analysis? How do we know the pores of the material are interconnected or isolated?

I have synthesized a series of porous organic polymers by tuning the linker length and observed a trend of variation of surface area (ultramicroporous, high surface area to mesoporous, low surface...

25 November 2018 3,962 2 View

Resuspend salivary proteins in 1X Coupling Buffer???

I have to enrich glycoproteins from salivary samples. I am using hydrazide resin. But it's very difficult to resuspend salivary proteins in 1X coupling buffer. Is there any solution for it?

07 September 2017 3,897 2 View

Why do we have strong spin orbit coupling only the surface electrons of topological insulator not for the bulk electrons?

is it possible to have strong spin orbit coupled electronic motion in 3D??

25 October 2016 3,756 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View