What would be the most suitable (and feasible) protocol for two viruses isolation from the same sample?

19 November 2015 1 4K Report

I've detected avian nephritis virus (RNA, nonenveloped) and avian adenovirus (DNA, nonenveloped) in the same feces sample. My aim is to isolate and propagate each one of these viruses in embryonated eggs or cell culture, but i'm afraid the fact that both are nonenveloped would be a problem. Could anybody suggest a protocol?

Daniel Ferreira de Lima Neto

Hi Leonardo, have you considered ultra centrifugation?

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC116360/

http://www.tandfonline.com/doi/pdf/10.1080/03079459508419108

Badges
Science topic

More Leonardo Caserta's questions See All

Experience Sampling Method (ESM) Solutions for Real-Time Voice Data Collection?

Hi Everyone, I am working on a research project focused on analyzing behavior and cognition in different environments and situations. I am looking for a solution that allows us to: Record...

29 June 2024 6,540 0 View

What is the best way to calculate the cavity's volume of macrocyles?

Does anyone know an unbiased way to define the cavity and volume of macrocycles, such as crown ethers, cyclodextrin, and similar molecules, using quantum chemistry software? All papers that I'm...

25 March 2024 5,265 8 View

How does the design of tourism-focused public space affect local communities?

Cities compete with each other in a globalised environment. How does this affect the design of urban space and the community?

04 March 2024 3,319 2 View

How to sort C2C12 and NSC-34 from a co-culture?

Hi, I need to do a co-culture with these two cell types and I would like to do a qPCR. Unfortunately I can't do a flow cytometry so I would use a magnetic beads separation system to isolate the...

19 February 2024 7,155 0 View

Hi guys, someone here knows how to calculate the FWHM from a FTIR spectra on Origin or Omnic softwares?

I have FTIR spectra and I need to calculate their FWHM. I tried many times on Origin, but never worked. I guess that is something related to the old version that I am using (8.5). Can you help...

09 February 2024 4,241 7 View

Repeated measurements ANOVA: how to interpret it?

I have read about the topic and I was wondering if anybody could help me to interpret the following description of repeated measurements I found in a manuscript (very detailed but still confuse in...

14 December 2023 4,028 3 View

Vegetation indices calculated at beginning of the crop cycle: remove or not soil pixels?

I have bi-weekly UAV flights in soybean trials and I was wondering about removing or not pixels classified as "soil" from the images to calculate veg. indices. Initially, I am calculating for of...

13 November 2023 1,838 1 View

How can I solve the runtime error running phonopy-gruneisen?

I am calculating the gruneisen parameter for a structure with 28 atoms in a 1x1x1 supercell and 224 in a 2x2x2 supercell, and I am having some issues with phonopy. When I try to run the...

10 October 2023 2,070 2 View

How can I solve warnings after running brm (brms package)?

I am running a phylogenetic logistic regression using brm from brms package. My data consists on a categorical predictor (values 0, 1 and 2) and a binary response variabe (values 1 and 2) plus...

06 August 2023 2,998 1 View

Are there any papers on the application of jerk equations (third-order differential equations) in time series forecasting within econometrics?

I am currently exploring advanced mathematical techniques within the field of econometrics and am particularly interested in understanding how third-order differential equations, commonly referred...

02 August 2023 9,473 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View