I am differentiating macrophages from THP-1 cells using PMA. Following PMA differentiation, I will be aiming to obtain M1 and M2a macrophages (by adding LPS, IL-4 etc).
Having read many publications regarding macrophages, I'm getting slightly confused. I am trying to select markers for flow cytometric panel to differentiate between different populations of macrophages e.g.
CD11b is a pan-macrophage marker which can be used to make a distinction between macrophages in general from THP-1 cells. Then I thought of using for instance CD86 and CD80 to select M1 macrophages. Similarly, I'd select CD163 and CD206 for M2a.
I'm not sure whether this is the best strategy to do so. I've seen multiple gating strategies and ways to make distinction between different populations of macrophages. I also understand they can be divided functionally or phenotypically. Any suggestions or good publications/protocols in this area would be much appreciated.
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