Regarding metagenomic contamination?

We have lab established culture setup of marine synechococcus PCC 7002 sp. and heterotrophic bacteria. Recently we did metagenomic analysis of it (sequencing was done by an outside reputed...

12 August 2020 187 11 View

How to find complete protein sequences (not partial) from NCBI database?

I want to generate a custom database of translated proteins involved in a particular process and then blast them against my predicted protein sequences from the metagenome . I have downloaded all...

12 July 2020 2,651 1 View

Can we collaborate on ongoign COVID-19 projects?

Hello all, I am a clinical epidemiologist with training in data analysis, manuscript writing and systematic review. Is there any group around where we can collaborate on your ongoing...

08 June 2020 3,831 48 View

How to identify a particular gene/gene cluster of interest from a sequenced genome?

We have sequenced Synechococcus sp. PCC 7002 genome. Already the same strain has been sequenced and annotated by many before. Whole-genome map is also available online. The gene of interest is...

17 March 2020 898 12 View

Is there any problem to have a absorbance value less than 0.1 in atomic absorption spectrophotometer ?

I am working on different heavy metals with a very low concentration (eg. less than 1ppm). please suggest some possible reasons. My absorption values are coming like 0.0023, 0.0036 etc.

27 March 2019 6,696 5 View

Which is the best/optimum preservative techniques for RNA preservation?

we want to carry out transcriptomics study of a mixed marine bacterial community. The experiment is designed as such that we have to preserve the samples for later transcriptomic studies...

26 February 2019 7,220 16 View

RNA extraction and gel electrophoresis ?

Dear all, I am trying to isolate RNA from bacteria for RNA seq. I have used fresh samples for extraction as well as 3 days stored (-80) samples. After gel electrophoresis I didnt observed 3...

04 December 2018 8,059 10 View

What are the possible reasons to decrease in wear rate?

Dear All, What may be the possible reasons to decrease in mass loss and wear rate with rise in load and temperature successively at a constant speed. Thank you.

26 November 2018 1,189 6 View

Is it possible to do aero acoustics analysis using Ansys Fluent?

How to do Aero acoustics analysis of the 2 D flow problem in Ansys fluent with k-epsilon model? What commands has to be given for this analysis in this commercial software?

31 May 2018 8,673 5 View

How to perform POD and DMD of transient problem?

I am working on Ansys fluent and I want to know how i can perform POD and DMD of my current flow problem over inclined flat plate at high angle of attack(in ansys fluent) in post processing state...

11 January 2018 5,871 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View