How do I extract 20 amino acids peptide in tissue matrix the charge is +4 ? Why is 5% trichloroacetic acid (TCA) needed to extract the peptide?

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Nick Gee

TCA is often used to precipitate proteins and, at an appropriate concentration, may selectively precipitate proteins over small peptides, giving a supernatant enriched for peptides.

Thaís Pedrete

You can also use other solvent to precipitate your protein, like acetonitrile or acetone. If you still have problems in isolating it, I suggest to perform a 2D gel or use some kind of tag.

Kolita Jinadasa

Article Validation of microwave-assisted sample extraction-high perf...